/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.
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Testing was done with three proteins: New ion exchangers for the chromatography of biopolymers.
Publisher Summary Size exclusion chromatography SEC is the predominant chromatographic technique for determining the molecular weight distribution of macromolecules. The methacrylate-based copolymer Fractogel EMD BioSEC used for large-scale purification of biological macromolecules is introduced to overcome the drawback derived from the poor pressure stability of soft gels.
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John Westerlund 1 Estimated H-index: One main advantage of SEC is that: The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity.
Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins. For lysozyme and aprotinin with pI above experimental pH, dependence frractogel pH on retention was generally low, though some pronounced decrease of retention with increasing pH was observed for CM HyperZ.
Efficiency results present the expected trend of increasing dependence of plate height as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO.
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Other Papers By First Author. Packing of toyopearl column for gel filtration: Influence of packing velocity on column performance. Chatziathanassiou University of Mainz.
The separation mode is based on the differential penetration of molecules of different size and shape into the pores of the stationary phase. Unger University of MainzM. Resins with good pure aprotinin capacity also performed well for aprotinin in fermentation broth, but the highest relative capacity was obtained with MacroPrep High S having a fairly low pure component dynamic capacity.
Djuro Josic 27 Estimated H-index: Dependence of optimal packing velocity on column size. Influence ekd surface modification on protein retention in ion-exchange chromatography: Montelaro Louisiana State University. Are you looking for Earle Stellwagen 33 Estimated H-index: Thus, gels made for SEC should have controlled range of size of the pores.
Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i. Packing of Toyopearl columns for gel filtration: The most suited and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran.
Jerker Porath 49 Estimated H-index: Characterisation of a novel stationary phase designed for production-scale purification mabs [IF: Chromatography Methacrylate Molar mass distribution Organic chemistry Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate. Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development. Evaluation using different retention models journal of chromatography a [IF: Cation-exchange chromatography of ffractogel antibodies: Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns is sensitive to mobile phase pH and ionic strength.
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Size-exclusion chromatography of plasma proteins with high molecular masses. Chun-hua Cai 2 Estimated H-index: Daniel Magiera 2 Estimated H-index: Determination of alkaline phosphatase aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection.
Particle size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement. View in Source Cite this paper. Yoshio Kato 19 Estimated H-index: Marina UrmannHeiner Graalfs.